Small interference of transcription factor Snail contribute to enhanced cisplatin sensitivity on human laryngeal resistant cancer cells

• Published in: Lin chuang er bi yan hou ke za zhi Vol. 32; no. 5; p. 350
• Main Authors: Yu, F, Gong, X R, Zhou, Y B, Liu, W
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2018
• Subjects: laryngeal cancer; drug resistance; small interference RNA; chemo-sensitivity; Snail; cisplatin
• ISSN: 1001-1781
• DOI: 10.13201/j.issn.1001-1781.2018.05.008

To study the relationship between transcription factor Snail and the sensitivity of cisplatin on human laryngeal resistant cancer cells. siRNA interference of Snail was transfected by small RNA interference technology. The interference efficiency on mRNA level were detected by RT-qPCR assay; the expression of Snail protein level was assessed by immunofluorescence. The inhibition ratio of different cisplatin concentration (0, 1, 2, 4, 8, 16 μg/ml) was detected by CCK-8 assay; the protein level of Snail, E-cadherin, MDR1were detected by Western blot assay. RT-qPCR assay show the expression of Snail on mRNA level was decreased to (67.85±9.50)% after transfection in Hep-2/CDDP cell( <0.05). Immunofluorescence show fluorescence intensity of si-Hep-2/CDDP group was reduced both in nucleus and cytoplasm; CCK-8 assay show the inhibitory ratio of transfected group was increased compared to negative control and Hep-2/CDDP group in different cisplatin concentration (0, 1, 2, 4, 8, 16 μg/ml) ( <0.05). Western blot assay