Systematic comparison of two kinds of Bufonis Venenum derived from different Bufo gargarizans subspecies based on metabolomics and antitumor activity

• Published in: Zhongguo zhongyao zazhi Vol. 48; no. 5; p. 1280
• Main Authors: Sun, Bo, Li, Ming-Li, Ding, Yao-Hua, Zhang, Yong, Xia, Bo, Guo, Sheng-Ya, Wang, Shi-Lu, Bian, Bao-Lin, Si, Nan, Zhao, Hai-Yu
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2023
• Subjects: Animals; Bufanolides - analysis; Bufonidae; Cell Line, Tumor; Chromatography, High Pressure Liquid; Quality Control; Zebrafish; active evaluation; Bufonis Venenum; Bufo gargarizans gargarizans; Bufo gararizans andrewsi; quality markers
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20220627.202

This paper compared the differences between two kinds of Bufonis Venenum produced by Bufo gargarizans gargarizans and B. gararizans andrewsi, and verified the rationality of the market value orientation of Bufonis Venenum based on the zebrafish mo-del. Twenty batches of Bufonis Venenum from Jiangsu province, Hebei province, Liaoning province, Jilin province, and Liangshan, Sichuan province, including B. gargarizans gargarizans and B. gararizans andrewsi, were collected. The UHPLC-LTQ-Orbitrap-MS combined with principal component analysis was used to compare the differences between two kinds of Bufonis Venenum. According to the limiting conditions of VIP>1, FC<0.5 or FC>2.0, and peak total area ratio>1%, 9 differential markers were determined, which were cinobufagin, cinobufotalin, arenobufagin, resibufogenin, scillaredin A, resibufagin, 3-(N-suberoylargininyl)-arenobufagin, 3-(N-suberoylargininyl)-marinobufagin, and 3-(N-suberoylargininyl)-resibufogenin. The content of 20 batches of Bufonis Venenum was determ