Effect of moxibustion on immune function homeostasis in rats with diarrhea irritable bowel syndrome based on SCF/c-kit signaling pathway

• Published in: Zhōngguó zhēnjiǔ Vol. 43; no. 2; p. 177
• Main Authors: Li, Kui-Wu, Chu, Hao-Ran, Ruan, Jing-Ru, Wang, Jiao-Jiao, Chen, Jin-Yu, Zhu, Shan-Shan, Zou, Ling, Liao, Lu-Min, Tong, Ting-Ting
• Format: Journal Article
• Language: Chinese
• Published: China 12.02.2023
• Subjects: Animals; Diarrhea; Homeostasis; Immunity; Immunoglobulin A; Immunoglobulin M; Interleukin-10; Interleukin-8; Irritable Bowel Syndrome - therapy; Maternal Deprivation; Moxibustion - methods; Rats; Rats, Sprague-Dawley; Receptor Protein-Tyrosine Kinases; Signal Transduction; Tumor Necrosis Factor-alpha; moxibustion; diarrhea irritable bowel syndrome; immune function; stem cell factor/tyrosine kinase receptor (SCF/c-kit) signaling pathway
• ISSN: 0255-2930
• DOI: 10.13703/j.0255-2930.20220108-k0007

To observe the effects of moxibustion on the stem cell factor (SCF)/tyrosine kinase receptor (c-kit) signaling pathway and immune function in rats with diarrhea irritable bowel syndrome (IBS-D), and to explore the mechanism of moxibustion for IBS-D. Among 52 young rats born from 6 healthy pregnant SPF rats, 12 rats were randomly selected into the normal group, and the remaining 40 rats were treated with the three-factor combination method of maternal separation, acetic acid enema and chronic restraint stress to establish the IBS-D rat model. Thirty-six rats with successful IBS-D model were randomly divided into a model group, a moxibustion group, and a medication group, 12 rats in each group. The rats in the moxibustion group were treated with suspension moxibustion at "Tianshu" (ST 25) and "Shangjuxu" (ST 37); the rats in the medication group were treated with intragastric administration of rifaximin suspension (150 mg/kg). All the treatments were given once a day for 7 consecutive days. The body mass, loose