Silencing long non-coding RNA HIF1A-AS2 inhibits proliferation, invasion and migration of cervical cancer cells in vitro

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 40; no. 11; p. 1615
• Main Authors: Deng, Rong, Zhang, Fuyun, Lei, Fuzhen, Liu, Wenjun, Liu, Sisun, Wang, Wenhua
• Format: Journal Article
• Language: Chinese
• Published: China 30.11.2020
• Subjects: Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Epithelial-Mesenchymal Transition - genetics; Female; Gene Expression Regulation, Neoplastic; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; RNA, Long Noncoding - genetics; RNA, Small Interfering - genetics; Uterine Cervical Neoplasms - genetics; epithelial-mesenchymal transition; long non-coding RNA HIF1A-AS2; tumor stem cell; cervical cancer
• ISSN: 1673-4254
• DOI: 10.12122/j.issn.1673-4254.2020.11.12

To explore the inhibitory effects of silencing long non-coding RNA (LncRNA) HIF1A-AS2 on epithelialmesenchymal transition (EMT) and tumor stem cell-like phenotype in cervical cancer cells. We designed 3 shRNA constructs for silencing HIF1A-AS2 in CaSki cells, and the shRNA with the strongest interference effect was selected for subsequent experiment. CaSki cells were transfected with shRNA-NC or Sh-HIF1A-AS2, and the changes in cell viability, invasion ability, EMT, expressions of EMT-related proteins, formation of cell spheres and expressions of stem cell markers were detected. Transfection with shRNA-NC and Sh-HIF1A-AS2 did not significantly affected the viability of CaSki cells ( > 0.05). Compared with the cells transfected with shRNA-NC, the cells transfected with Sh- HIF1A-AS2 showed significantly reduced invasion ability, expressions of vimentin N-cadherin, and cell sphere formation ability. HIF1A-AS2 silencing obviously lowered the rate of ABCG2-positive cells, significantly reduced the mRNA and protei