Mechanism of Notoginseng Radix et Rhizoma in regulating PI3K/Akt/mTORC1-mitochondrial energy metabolism to treat chronic renal failure in rats with blood stasis syndrome

• Published in: Zhongguo zhongyao zazhi Vol. 49; no. 7; p. 1888
• Main Authors: Liu, Xiao-Yu, Huang, Zhi-Min, Qiu, Shao-Bin, Qiu, Fei, Huang, Xiao-Ying, Jiang, Yi-Ni, Lin, Ai-Tao, Zhang, Zhi-Ying, Tang, Yu, Wu, Jin-Yu
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2024
• Subjects: Animals; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - pharmacology; Energy Metabolism - drug effects; Humans; Kidney - drug effects; Kidney - metabolism; Male; Mechanistic Target of Rapamycin Complex 1 - metabolism; Mitochondria - drug effects; Mitochondria - metabolism; Panax notoginseng - chemistry; Phosphatidylinositol 3-Kinases - genetics; Phosphatidylinositol 3-Kinases - metabolism; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; Rats; Rats, Sprague-Dawley; Renal Insufficiency - drug therapy; Renal Insufficiency - metabolism; Rhizome - chemistry; Signal Transduction - drug effects; blood stasis; Notoginseng Radix et Rhizoma; mitochondrial energy metabolism; renal fibrosis; PI3K/Akt/mTORC1
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20231009.403

This study observed the effects of Notoginseng Radix et Rhizoma on the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin complex 1(mTORC1) signaling pathway and mitochondrial energy metabolism in the rat model of adriamycin-induced renal fibrosis with blood stasis syndrome to explore the mechanism of Notoginseng Radix et Rhizoma in protecting the kidney. Thirty male rats with adriamycin-induced renal fibrosis were randomized into model, low-, medium-, and high-dose Notoginseng Radix et Rhizoma, and positive control groups(n=6). Six clean SD male rats were selected into the normal group. The normal group and model group were administrated with normal saline, and other groups with corresponding drugs. After 8 weeks of treatment, the renal function, renal pathology, adenosine triphosphate(ATP) levels, Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase activities, and the protein levels of ATP5B, mTORC1, 70 kDa ribosomal protein S6 kinase(P70S6K), P85, Akt, p-Akt, and SH2-containing