Autophagic activity of piperine on small intestine in dementia model mice with Parkinson's disease

• Published in: Zhongguo zhongyao zazhi Vol. 45; no. 21; p. 5238
• Main Authors: Li-Ping, Huang, Xiao-Qin, Zhong, Qing, Luo, Qin-Xin, Zhang, Min-Zhen, Deng
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2020
• Subjects: Alkaloids; Animals; Autophagy; Benzodioxoles; Dementia; Intestine, Small; Male; Mice; Parkinson Disease; Piperidines; Polyunsaturated Alkamides; piperine; autophagy; inflammation; Parkinson′s disease with dementia; miRNA-99a; α-synuclein
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20200624.408

This article is to investigate the effect of piperine on the small intestine of mice with Parkinson's disease with dementia(PDD). Ninety-six C57 BL/6 mice of SPF grade were randomly divided into 8 groups(male, 12 in each group): normal group, model group, autophagy inhibitor group(6-amino-3-methylpurine, 3 MA, 30 mg·kg~(-1)), autophagy activator group(rapamycin, 1 mg·kg~(-1)), low, medium, and high dose piperine groups(10, 20, 40 mg·kg~(-1)), and medopar group(112.5 mg·kg~(-1)). Except for the normal group, mice in each group were injected subcutaneously with reserpine(0.1 mg·kg~(-1)) once every 48 hours for 40 days. In addition, on the 20 th day of administration, except for the normal group, the mice in the other groups were subjected to bilateral common carotid artery occlusion to finally prepare PDD models. At the same time, each group was given the corresponding drug treatment once a day for 40 days. After the last administration, the behavioral changes of mice were observed by autonomic activity experim