Reconstruction of Hematopoietic Function of Human Placental Hematopoietic Stem Cells in Non-Lethally Radiated Mice

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 27; no. 6; p. 1986
• Main Authors: Ji, Xia-Yun, Zhu, Feng-Cheng, Li, Tian-Yu, Zhou, Xin, Wang, Hao
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2019
• Subjects: Animals; Bone Marrow Cells; Female; Flow Cytometry; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells; Humans; Mice; Mice, Inbred C57BL; Pregnancy
• ISSN: 1009-2137
• DOI: 10.19746/j.cnki.issn.1009-2137.2019.06.045

To investigate the effect of human placental hematopoietic stem cells (PHSCs) on hematopoietic reconstruction in non-lethally irradiated mice. Human placental HSCs were extracted by mechanical method combined with zymolysis and were identified by flow cytometry and colony formationtest. Twenty-five NOG mice were divided randomly into 4 groups: the blank control group (n=5), the irradiated group (n=4), the low dose PHSC group (n=8) and the high dose PHSC group (n=8). The mice in the irradiated, the low dose and the high dose PHSC groups were irradiated with X-rays at dose 1 Gy (100 cGy/min) under sterile condition. The mice in the low dose PHSC group and the high dose PHSC group were injected intravenously with 0.1 ml human placental HSC in dose of 2×10 and 1×10 , respectively. The mice in the blank control and the irradiated group were injected with the same volume of saline. The mice were weighed weekly, and the changes of body weight were calculated. The peripheral blood was collected from each group at 4,