Effects of Rehmanniae Radix and Rehmanniae Radix Praeparata on proteomics and autophagy in mice with type 2 diabetes mellitus induced by high-fat diet coupled with streptozotocin

• Published in: Zhongguo zhongyao zazhi Vol. 48; no. 6; p. 1535
• Main Authors: Yan, Jing-Ning, Liu, Xiao-Qin, Meng, Xiang-Long, Ren, Ke-le, Wu, Xue-Min, Zhang, Hao, Wang, Hai-Qin, Wang, Hong-Liang, Sheng, Qi, Li, Bin, Zhang, Ding-Bang, Chen, Hong-Zhou, Zhang, Fa-Yun, Li, Ming-Hao, Zhang, Shuo-Sheng
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2023
• Subjects: Animals; Autophagy; Diabetes Mellitus, Type 2 - drug therapy; Diabetes Mellitus, Type 2 - genetics; Diet, High-Fat - adverse effects; Inflammation; Mammals; Mice; Proteomics; Streptozocin - pharmacology; TOR Serine-Threonine Kinases; type 2 diabetes mellitus; Rehmanniae Radix Praeparata; autophagy; proteomics; Rehmanniae Radix
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20220901.301

To compare the pancreatic proteomics and autophagy between Rehmanniae Radix-and Rehmanniae Radix Praeparata-treated mice with type 2 diabetes mellitus(T2DM). The T2DM mouse model was established by high-fat diet coupled with streptozotocin(STZ, intraperitoneal injection, 100 mg·kg~(-1), once a day for three consecutive days). The mice were then randomly assigned into a control group, low-(5 g·kg~(-1)) and high-dose(15 g·kg~(-1)) Rehmanniae Radix groups, low-(150 mg·kg~(-1)) and high-dose(300 mg·kg~(-1)) catalpol groups, low-(5 g·kg~(-1)) and high-dose(15 g·kg~(-1)) Rehmanniae Radix Praeparata groups, low-(150 mg·kg~(-1)) and high-dose(300 mg·kg~(-1)) 5-hydroxymethyl furfuraldehyde(5-HMF) groups, and a metformin(250 mg·kg~(-1)) group. In addition, a normal group was also set and each group included 8 mice. The pancreas was collected after four weeks of administration and proteomics tools were employed to study the effects of Rehmanniae Radix and Rehmanniae Radix Praeparata on protein expression in the pancreas