Effects of altered IFNAR expression on CD4+ CD25+ treg cell dysfunction in patients with systemic lupus erythematosus
To explore the role of type I interferon (IFNalpha) on the function of CD4+ CD25+ regulatory T (Treg) cells in patients with systemic lupus erythematosus (SLE). Twenty patients with newly-onset active SLE and 20 healthy controls were recruited in this study. The expressions of type I interferon a re...
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Published in | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 46; no. 2; p. 209 |
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Main Authors | , , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
01.03.2015
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Subjects | |
Online Access | Get more information |
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Summary: | To explore the role of type I interferon (IFNalpha) on the function of CD4+ CD25+ regulatory T (Treg) cells in patients with systemic lupus erythematosus (SLE).
Twenty patients with newly-onset active SLE and 20 healthy controls were recruited in this study. The expressions of type I interferon a receptor (IFNAR) on Treg cells were analyzed using flow cytometry. CD4+ CD25+ Treg cells were purified by magnetic-activated cell sorter (MACS). The function of these cells was assessed in vitro with or without IFNalpha (500 U/mL). The effect of exogenous IFNalpha on the apoptosis of Treg cells and the expression level of FOXP3, CTLA-4, and pAKT in Treg cells were analyzed using flow cytometry. Results The expression levels of IFNAR1 on CD4+ CD25+ Treg cells were significantly higher in the SLE patients than in the healthy controls (P=0.0006). There was a positive correlation between the expression levels of IFNAR on Treg cells and the SLEDAI scores in the SLE patients. Exogenous IFNalpha impaired the suppressive cap |
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ISSN: | 1672-173X |