RNA immunoprecipitation technique for Drosophila melanogaster S2 cells

RNA-binding proteins play an important role in RNA metabolism, especially in mRNA biogenesis and subsequent expression patterns regulation. RNA immunoprecipitation (RIP) is a powerful tool for detecting protein-RNA associations. In this paper, we briefly cover the history of this method for analyzin...

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Published inMolekuliarnaia biologiia Vol. 51; no. 1; p. 85
Main Authors Kachaev, Z M, Gilmutdinov, R A, Kopytova, D V, Zheludkevich, A A, Shidlovskii, Y V, Kurbidaeva, A S
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.2017
Subjects
PABP
RNA-binding proteins
Drosophila melanogaster
S2 cells
RNA immunoprecipitation
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Summary:RNA-binding proteins play an important role in RNA metabolism, especially in mRNA biogenesis and subsequent expression patterns regulation. RNA immunoprecipitation (RIP) is a powerful tool for detecting protein-RNA associations. In this paper, we briefly cover the history of this method for analyzing RNA-protein interactions and reviewing a number of modifications of the RIP technique. We also present an adjusted RIP protocol that was modified for Drosophila S2 cell culture. The use of this protocol allows one to perform the efficient precipitation of RNA-protein complexes and harvest RNA in amounts that are sufficient for its downstream analysis.
ISSN:0026-8984
DOI:10.7868/S0026898416060082