High glucose induces transdifferentiation of HK-2 human renal tubular epithelial cells by activating reactive oxygen species-mediated NF-kappa B signaling pathway

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 35; no. 4; p. 313
• Main Authors: Zhang, Lei, Jin, Hua, Wang, Yiping, Wang, Dong, Li, Zhuoya
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2019
• Subjects: Cell Line; Cell Transdifferentiation; Culture Media; Epithelial Cells - cytology; Glucose; Humans; Kidney Tubules - cytology; NF-kappa B - metabolism; Pyrrolidines; Reactive Oxygen Species - metabolism; Signal Transduction; Thiocarbamates
• ISSN: 1007-8738

Objective To explore the mechanism of transdifferentiation of human renal tubular epithelial cells induced by high glucose based on reactive oxygen species (ROS)/NF-κB signaling pathway. Methods HK-2 normal human proximal tubular epithelial cells were randomly divided into blank control group, osmotic pressure control group, high glucose group and pyrrolidine dithiocarbamate (PDTC) group. Cell morphology was observed by phase contrast microscope. Cell viability was measured by MTT assay. Flow cytometry was used to detect intracellular ROS content. Malondialdehyde (MDA) content and superoxide dismutase activity were tested by ELISA. Western blot analysis was used to examine the protein levels of NF-κBp65, phosphorylated IκBα (p-IκBα) and IKKα, monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1). The expressions of NF-κBp65, epithelial cadherin (E-cadherin) and alpha smooth muscle actin (α-SMA) were assessed by immunocytochemistry. Results In high glucose group, the cells b