Molecular-genetic characterization of canine and rangiferine brucella isolates from different regions of Russia

Comparative molecular-genetic characterization of Brucella isolates from dogs and reindeers in Russia by molecular-genetic typing methods. 19 canine and 2 rangiferine Brucella isolates were studied by molecular typing methods based on PCR for differential species and biovar specific molecular target...

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Published inMolekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 4; p. 28
Main Authors Kulakov, Iu K, Tsirel'son, L E, Zheludkov, M M
Format Journal Article
LanguageRussian
Published Russia (Federation) 2012
Subjects
Animals
Bacterial Typing Techniques - methods
Brucella - genetics
Brucella - isolation & purification
Brucellosis - epidemiology
Brucellosis - genetics
Brucellosis - microbiology
Brucellosis - veterinary
Dogs
Humans
Polymerase Chain Reaction - methods
Reindeer - genetics
Reindeer - microbiology
Russia - epidemiology
Russia
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Summary:Comparative molecular-genetic characterization of Brucella isolates from dogs and reindeers in Russia by molecular-genetic typing methods. 19 canine and 2 rangiferine Brucella isolates were studied by molecular typing methods based on PCR for differential species and biovar specific molecular targets and MLVA (multiple locus variable number tandem repeats analysis) using primers to 12 known variable loci. Using PCR for differential molecular targets, canine Brucella isolates were characterized as B. canis and rangiferine isolates as B. suis biovar 4. MLVA revealed 5 identical and 7 variable MLVA loci. Using the dendrogram. all the isolates on the data of 12 loci were classified into the close related cluster. On the other hand, high discrimination power of MLVA with a resulting Hunter and Gaston discriminatory index (HGDI) of 0.9842 was shown to reveal genetic diversity for the isolates of 17 MLVA genotypes. B. canis and B. suis isolates from different geographical regions in Russia were genetically close related, thereby confirming known genetic relationship between these species. Related MLVA genotypes of isolates were connected to certain regions of preliminary isolation in Russia. To improve the system ofbrucellosis surveillance in Russia MLVA typing of more canine and rangiferine Brucella isolates having epidemiological danger for humans is required to be studied.
ISSN:0208-0613