A new cucurbitane glycoside from Picrorhiza scrophulariiflora

Chemical constituents from the ethanol extract of Picrorhiza scrophulariiflora were isolated and purified by column chromatography. Their structures were identified by HR-MS, 1D and 2D-NMR, and their cytotoxicity was assessed by CCK-8 assay. Four compounds were isolated and identified as follows: 2β...

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Published inZhongguo zhongyao zazhi Vol. 49; no. 7; p. 1882
Main Authors Ji, Hai-Jie, Wang, Shu, Wang, Dong-Mei, Hao, Shu-Lan, Ma, Xiang-Xue, Tong, Li-Guo, Lu, Bo-Cheng, Li, Mei-Fang, Zhou, Wen-Jing, Shi, Mei-Jun, Wang, Xi-Xing, Tang, Xu-Dong
Format Journal Article
LanguageChinese
Published China 01.04.2024
Subjects
Cell Line, Tumor
Drugs, Chinese Herbal - chemistry
Glycosides - chemistry
Glycosides - isolation & purification
Humans
Magnetic Resonance Spectroscopy
Molecular Structure
Picrorhiza - chemistry
Triterpenes
cucurbitane glycosides
Picrorhiza scrophulariiflora
cytotoxicity
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Summary:Chemical constituents from the ethanol extract of Picrorhiza scrophulariiflora were isolated and purified by column chromatography. Their structures were identified by HR-MS, 1D and 2D-NMR, and their cytotoxicity was assessed by CCK-8 assay. Four compounds were isolated and identified as follows: 2β-D-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosterol-5,25-diene-22-one(1), 2β-D-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosta-5,24-diene-22-one(2), 25-acetoxy-2β-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosta-5-ene-22-one(3) and 25-acetoxy-2β-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosta-5,23-(E)-diene-22-one(4). Compound 1 represents a new cucurbitane glycoside. The half inhibitory concentrations of the 4 compounds exceeded 100 μmol·L~(-1) against four tumor cell lines, indicating no significant cytotoxicity.
ISSN:1001-5302
DOI:10.19540/j.cnki.cjcmm.20240108.201