Establishment and Evaluation of a Resazurin-Based Microdilution Assay for Microbial Sensitivity Test of Neisseria gonorrhoeae

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 55; no. 1; pp. 198 - 203
• Main Authors: Li, Menghuan, Yang, Guiqin, Wang, Youwei, Yong, Gang, Wang, Hongren, Bie, Mingjiang, Wang, Guoqing
• Format: Journal Article
• Language: Chinese
• Published: China 20.01.2024
• Subjects: Microbial sensitivity test; Resazurin-based microdilution assay; Neisseria gonorrhoeae
• ISSN: 1672-173X
• DOI: 10.12182/20240160209

To establish and evaluate a microbial sensitivity test method for based on resazurin coloration. Based on the broth microdilution method, resazurin was added as a live bacteria indicator. WHO G, a WHO gonococcal reference strain, was used to optimize the incubation time for resazurin-stained bacteria and the color change was visually observed to obtain the results. Agar dilution method (the gold standard) and resazurin-based microdilution assay were used to determine the minimum inhibitory concentration (MIC) of azithromycin, ceftriaxone, and spectinomycin for 3 reference strains and 32 isolates of . The results were analyzed based on essential agreement (EA), which reflected the consistency of the MIC values, category agreement (CA), which reflected the consistency in the determination of drug resistance, intermediary, and sensitivity, very major error (VME), which reflected false sensitivity, and major error (ME), which reflected pseudo drug resistance, to evaluate the accuracy of resazurin-based microdilut