Diagnostic value of MDM2 RNA in situ hybridization in atypical lipomatous tumor/well-differentiated liposarcoma and dedifferentiated liposarcoma

• Published in: Zhonghua bing li xue za zhi Vol. 51; no. 3; p. 190
• Main Authors: Chen, C, He, X, Jing, W Y, Qiu, Y, Chen, M, Luo, T Y, Liu, X Y, Chen, H J, Zhang, H Y, Bu, H
• Format: Journal Article
• Language: Chinese
• Published: China 08.03.2022
• Subjects: Biomarkers, Tumor - genetics; Gene Amplification; Humans; In Situ Hybridization, Fluorescence; Liposarcoma - diagnosis; Liposarcoma - genetics; Proto-Oncogene Proteins c-mdm2 - genetics; RNA
• ISSN: 0529-5807
• DOI: 10.3760/cma.j.cn112151-20211029-00785

To investigate the value of MDM2 RNA in situ hybridization (RNA-ISH) in diagnosing atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) and dedifferentiated liposarcoma (DDL). A total of 26 ALT/WDL/DDLs diagnosed from March 2017 to May 2019 in West China Hospital, Sichuan University, Chengdu, China and 18 control cases were included. MDM2 RNA-ISH was performed on all samples and compared with the fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) regarding their performance in detecting MDM2. All samples were detected successfully using the three methods. Among 26 ALT/WDL/DDLs, all cases showed MDM2 amplification and positivity for MDM2 RNA-ISH (26/26, 100%). Twenty-four (24/26, 92.3%) of the 26 tested cases were positive for MDM2 IHC while two of them were negative. Eighteen control cases were all negative for MDM2 FISH and RNA-ISH, and 15 (15/18) cases were negative for MDM2 IHC. The sensitivity and specificity of RNA-ISH were both 100%, and those of MDM2 IHC were 92.3%