Diosgenin alleviates NAFLD induced by a high-fat diet in rats via mTOR/SREBP-1c/HSP60/MCAD/SCAD signaling pathway

• Published in: Zhongguo zhongyao zazhi Vol. 48; no. 19; p. 5304
• Main Authors: Chen, Su-Wen, Yin, Guo-Liang, Song, Chao-Yuan, Meng, De-Cheng, Yu, Wen-Fei, Zhang, Xin, Feng, Ya-Nan, Liang, Peng-Peng, Zhang, Feng-Xia
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2023
• Subjects: Animals; Body Weight; Chaperonin 60 - metabolism; Chaperonin 60 - pharmacology; Chaperonin 60 - therapeutic use; Diet, High-Fat - adverse effects; Diosgenin - metabolism; Lipid Metabolism; Liver; Male; Mammals - genetics; Mammals - metabolism; Non-alcoholic Fatty Liver Disease - etiology; Non-alcoholic Fatty Liver Disease - genetics; Rats; Rats, Sprague-Dawley; RNA, Messenger - metabolism; Signal Transduction; Simvastatin - metabolism; Simvastatin - pharmacology; Simvastatin - therapeutic use; Sterol Regulatory Element Binding Protein 1 - metabolism; TOR Serine-Threonine Kinases - genetics; TOR Serine-Threonine Kinases - metabolism; Triglycerides; mitochondrial function; lipid synthesis; non-alcoholic fatty liver disease; fatty acid β oxidation; diosgenin
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20230601.705

This study aims to observe the effects of diosgenin on the expression of mammalian target of rapamycin(mTOR), sterol regulatory element-binding protein-1c(SREBP-1c), heat shock protein 60(HSP60), medium-chain acyl-CoA dehydrogenase(MCAD), and short-chain acyl-CoA dehydrogenase(SCAD) in the liver tissue of the rat model of non-alcoholic fatty liver disease(NAFLD) and explore the mechanism of diosgenin in alleviating NAFLD. Forty male SD rats were randomized into five groups: a control group, a model group, low-(150 mg·kg~(-1)·d~(-1)) and high-dose(300 mg·kg~(-1)·d~(-1)) diosgenin groups, and a simvastatin(4 mg·kg~(-1)·d~(-1)) group. The rats in the control group were fed with a normal diet, while those in the other four groups were fed with a high-fat diet. After feeding for 8 weeks, the body weight of rats in the high-fat diet groups increased significantly. After that, the rats were administrated with the corresponding dose of diosgenin or simvastatin by gavage every day for 8 weeks. The levels of triglyceri