Biocompatibility of Fe(3)O(4) nanoparticles evaluated by in vitro cytotoxicity assays using normal, glia and breast cancer cells

• Published in: Nanotechnology Vol. 21; no. 7; p. 75102
• Main Authors: Ankamwar, B, Lai, T C, Huang, J H, Liu, R S, Hsiao, M, Chen, C H, Hwu, Y K
• Format: Journal Article
• Language: English
• Published: England 19.02.2010
• Subjects: Amino Acids - pharmacology; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Breast - cytology; Breast - drug effects; Breast - metabolism; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell Line; Cell Line, Tumor; Cell Survival - drug effects; Female; Ferric Compounds - chemistry; Ferric Compounds - pharmacology; Humans; Materials Testing - methods; Metal Nanoparticles - chemistry; Microscopy; Neuroglia - cytology; Neuroglia - drug effects; Neuroglia - metabolism; Quaternary Ammonium Compounds - pharmacology; Surface-Active Agents - pharmacology
• ISSN: 1361-6528
• DOI: 10.1088/0957-4484/21/7/075102

In order to reveal the biocompatibility of Fe(3)O(4) nanoparticles and bipolar surfactant tetramethylammonium 11-aminoundecanoate cytotoxicity tests were performed as a function of concentration from low (0.1 microg ml(-1)) to higher concentration (100 microg ml(-1)) using various human glia, human breast cancer and normal cell lines. Cytotoxicity tests for human glia (D54MG, G9T, SF126, U87, U251, U373), human breast cancer (MB157, SKBR3, T47D) and normal (H184B5F5/M10, WI-38, SVGp12) cell lines exhibited almost nontoxicity and reveal biocompatibility of Fe(3)O(4) nanoparticles in the concentration range of 0.1-10 microg ml(-1), while accountable cytotoxicity can be seen at 100 microg ml(-1). The results of our studies suggest that Fe(3)O(4) nanoparticles coated with bipolar surfactant tetramethylammonium 11-aminoundecanoate are biocompatible and promising for bio-applications such as drug delivery, magnetic resonance imaging and magnetic hyperthermia.