The effects of nm23-H1 gene cell transfection on abilities of invasion, adhesion and chemotherapeutic sensitivity of bladder cancer T-24 cell line

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 38; no. 5; p. 788
• Main Authors: Cui, Shu, Tang, Tie-long, Li, Xiang, Li, Hong
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2007
• Subjects: Cell Adhesion; Cell Line, Tumor; Cisplatin - pharmacology; Drug Resistance, Neoplasm; Genetic Vectors; Humans; Neoplasm Metastasis; NM23 Nucleoside Diphosphate Kinases - genetics; Plasmids; Transfection; Urinary Bladder Neoplasms - drug therapy; Urinary Bladder Neoplasms - genetics
• ISSN: 1672-173X

To investigate the transfection and expression of nm23-H1 gene in human bladder cancer cell line T-24, and to find out whether nm23-H1 could affect the metastases ability and the chemotherapeutic sensitivity of T-24 cell line. By electroporation way, the pCMV-Bam-neo-nm23-H1 plasmid was transferred into bladder cancer line T-24, and the nm23-H1 vector-producer cell line T-24/nm23-H1 was isolated with G418 selection. The expression of nm23-H1 gene q,as detected by immunohistochemistry. The T-24 invasion, adherence and migration abilities between transfected and non-transfected cell lines were detected by transwell-room and wash techniques. The difference or change to the sensitivity of chemotherapeutic drug before and after cell transfected was evaluated by MTT. The stable expression of nm23-H1 was obtained by pCMV-Bam-Neo system with G418. The expression of nucleoside diphosphate kinase-A (NDPKA) was observed for significant difference between transfected and non-transfected T-24 cell lines; The invasive, adh