Influenza virus reproduction in the MDCK cells adapted to growth in serum-free Hybris-2 medium

Whether the MDCK cell line might adapt to grow in serum-free Hybris-2 medium and influenza viruses might be reproduced in the adapted cells was studied. Seventeen passages using the Hybris-2 medium yielded cells adapted to growth in this medium (MDCK-BS). The reproduction of influenza A (H1N1 and H3...

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Published inVoprosy virusologiĭ Vol. 55; no. 5; p. 47
Main Authors Podcherniaeva, R Ia, Baklanova, O V, Glushchenko, L A, Isaeva, E I, Chestkov, V V, Tabakov, V Iu, Mikhaĭlova, G R
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.2010
Subjects
Animals
Cell Line
Culture Media, Serum-Free
Dogs
Influenza A virus - growth & development
Influenza B virus - growth & development
Virus Cultivation - methods
Virus Replication
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Summary:Whether the MDCK cell line might adapt to grow in serum-free Hybris-2 medium and influenza viruses might be reproduced in the adapted cells was studied. Seventeen passages using the Hybris-2 medium yielded cells adapted to growth in this medium (MDCK-BS). The reproduction of influenza A (H1N1 and H3N2) and B viruses versus the cells cultured in Eagle's medium was studied. The laboratory strain of influenza A/Aichi/1/68 (H3N2) and the strain B/Ohio/01/05 of influenza B in equal titers were shown to be reproduced in both control cells on Eagle's medium and MDCK-BS cells adapted to growth in the Hybris-2 medium. The reproduction of the strains A/Brisbane/10/07 (H3N2) and A/Solomon Islands/3/06 (H1N1) was less active in the MDCK cells. Each strain of influenza viruses displayed varying infective activities. The developed serum-free Hybris-2 medium may be used for cultivation of monolayer continuous MDCK cells and for their reproduction of influenza A and B viruses.
ISSN:0507-4088