The cell cycle regulator p130 and beta-catenin form a complex in mesenchymal stem cells

Suppressor complex p130/E2f4 inhibits transcription of multiple genes proteins regulating cell cycle progression and induces cell cycle arrest at G0/G1 required for induction of cell differentiation in cells of many tissues in vivo and various cell lineages in vitro. We found here that, in mesenchym...

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Published inT͡S︡itologii͡a Vol. 53; no. 2; p. 107
Main Authors Petrov, N S, Zhidkova, O V, Zenin, V V, Rozanov, Iu M, Popov, B V
Format Journal Article
LanguageRussian
Published Russia (Federation) 2011
Subjects
Animals
beta Catenin - genetics
beta Catenin - metabolism
Cell Cycle - physiology
Cell Line, Tumor
E2F4 Transcription Factor - genetics
E2F4 Transcription Factor - metabolism
Hepatocytes - cytology
Hepatocytes - metabolism
Humans
Male
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Mice
Multiprotein Complexes - genetics
Multiprotein Complexes - metabolism
Phosphorylation - physiology
Retinoblastoma-Like Protein p130 - genetics
Retinoblastoma-Like Protein p130 - metabolism
Signal Transduction - physiology
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Summary:Suppressor complex p130/E2f4 inhibits transcription of multiple genes proteins regulating cell cycle progression and induces cell cycle arrest at G0/G1 required for induction of cell differentiation in cells of many tissues in vivo and various cell lineages in vitro. We found here that, in mesenchymal stem cells, (MSC) activation of the Wnt/beta-catenin signal pathway induced by MSC coculture with the A-549 cell line or by growth in the medium containing Li+ ions, which resulted in the accumulation of active forms of the p130, E2f4 and beta-catenin, was not coupled with inhibition of cell cycle progression. Cell cycle synchronization of the MSC induced by thymidine and nocodazol was not resulted in change of the levels and phosphorylation pattern of the p130 in contrast to mouse hepatocytes and T98G cells which showed accumulation of the p130 form p1 and p2 in quiescence and form p3 under active proliferative. Antibody to p130 precipitated from extracts of MSC activated by Li+ ions beta the p130 form 2 and hyperphosphorilated beta-catenin. The results obtained suggest that Gsk3beta, p130 and beta-catenin form in MSC a complex the functional role of which may be associated with activation of differentiation not coupled to cell cycle arrest.
ISSN:0041-3771