Survival of probiotic microorganisms in the conditions in vitro imitating the process of human digestion

• Published in: Ėksperimental'nai͡a︡ i klinicheskai͡a︡ gastroėnterologii͡a no. 3; p. 6
• Main Authors: Darmov, I V, Chicherin, I Iu, Pogorel'skiĭ, I P, Lundovskikh, I A
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2011
• Subjects: Antibiosis; Bacillus - growth & development; Bacillus - isolation & purification; Bacteriological Techniques; Coculture Techniques; Digestion - physiology; Humans; Intestines - microbiology; Lactobacillus - growth & development; Lactobacillus - isolation & purification; Models, Biological; Probiotics; Stomach - microbiology
• ISSN: 1682-8658

Assessment of survival bifidobacteria and lactobacteria under the conditions in vitro, simulating digestion in human stomach and intestine, and study of survival probiotic and indigenous microorganisms in co-cultivation on solid nutrient medium. Probiotic microorganisms from commercial preparations Bifidobacterin and Lactobacterin, clinical isolates lactobacillus (Lactobacillus acidophilus No 1, L. brevis No 2) were used in experiments. Survival study of probiotic microorganisms was performed on a model in vitro, simulating the process of digestion in the human body. Assessment of the relationship of probiotic microorganisms and indigenous microorganisms was carried out in co-cultivation in vitro on solid nutrient medium. A significant reduction in the number of viable probiotic microorganisms during their incubation in model media was set as well as suppression of probiotic microorganisms growth by cultures of a clinical strains of lactobacillus, corresponding to biocompatibility by type "host against probiotic". While choosing probiotics in the treatment of dysbacterioses the character of relationship between probiotic microorganisms and indigenous microorganisms of a patient is recommended to be preliminarily tested. Also microorganisms of own microflora should be stimulated using modern prebiotics.