Uptake1 inibition in reperfusion: noradrenalin dynamic and myocardiola infraction size

• Published in: Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova Vol. 96; no. 2; p. 101
• Main Authors: Naumenko, S E, Latysheva, T V, Gilinskiĭ, M A
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.02.2010
• Subjects: Animals; Desipramine - pharmacology; Enzyme Inhibitors - pharmacology; Male; Myocardial Infarction - metabolism; Myocardial Infarction - pathology; Myocardium - metabolism; Myocardium - pathology; Norepinephrine - metabolism; Rats; Reperfusion
• ISSN: 0869-8139

To determine the effect of uptake 1 inhibition in reperfusion after myocardial ischcmia upon noradrenalin dynamic and myocardial infarction size, 14 male Wistar rats anesthetized with urethane were randomly divided into 2 groups and subjected to 30 min coronary occlusion and subsequent 120 min reperfusion. In control group (n = 7) 1 ml of Ringer's solution was administered intravenously at the beginning of reperfusion. In experimental group (n = 7) instead of Ringer's solution 0.8 mg/kg of desipramine was used. Myocardial interstitial noradrenalin levels were measured using a microdialysis technique. Infarction size was determined by triphenyltetrazolium chloride staining and was related to the area at risk. Desipramine increased infraction size from 32.0 +/- 3.1 % (control group) to 46.1 +/- 3.4% (p = 0.06). Interstitial noradrenalin in experimental group did not decrease during 120 min reperfusion (p < 0.05 vs. control). The data obtained suggest that uptake 1 inhibition in reperfusion after myocardial ischemia increases myocardial infarction size through increased interstitial noradrenalin.