Effect of tagalsin on p53 and Bcl-2 expression in hepatoma H(22) tumor-bearing mice

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 33; no. 7; p. 499
• Main Authors: Song, Xiu-qi, Guo, Yun-liang, Wang, Bing-gao, Sun, Shao-jie, Yao, Ru-yong
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2011
• Subjects: Animals; Body Weight - drug effects; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Line, Tumor; Diterpenes - pharmacology; Dose-Response Relationship, Drug; Drugs, Chinese Herbal - pharmacology; Female; Gene Expression Regulation, Neoplastic; Humans; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Mice; Neoplasm Transplantation; Plants, Medicinal - chemistry; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; Random Allocation; Rhizophoraceae - chemistry; RNA, Messenger - metabolism; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism
• ISSN: 0253-3766

To explore the effect and mechanism of tagalsin on hepatoma cells. The animal models were established by transplanting H(22) mouse hepatoma cells to mouse liver, and ten days later the mice were randomly divided into five groups: blank group, carmofur positive group and tagalsin groups, including low-dose, middle-dose and high-dose groups. Then medicine or oil was given to the mice by gastric gavage in consecutive 5 days with a 2-days interval as a course of treatment, two courses in all. All mice were killed at 24 hours after medication, and the survival period, ascites conditions, aggressive conditions intra- or extra-liver, weight changes, tumor volume and spleen index of the tumor-bearing mice were observed. Pathological changes of the tumors were examined. Apoptotic factors p53 and Bcl-2 protien and mRNA were detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). tagalsin inhibited the hepatoma growth effectively without influencing spleen index to some extent. The