Tyrosine hydroxylase, interleukin-1beta and tumor necrosis factor-alpha are overexpressed in peripheral blood mononuclear cells from schizophrenia patients as determined by semi-quantitative analysis

• Published in: Psychiatry research Vol. 176; no. 1; pp. 1 - 7
• Main Authors: Liu, Liang, Jia, Fujun, Yuan, Guozhen, Chen, Zaohuo, Yao, Jianjun, Li, Hengfen, Fang, Chunxia
• Format: Journal Article
• Language: English
• Published: Ireland 30.03.2010
• Subjects: Adult; Female; Gene Expression Regulation - physiology; Humans; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - metabolism; Male; Psychiatric Status Rating Scales; RNA, Messenger - metabolism; Schizophrenia - immunology; Schizophrenia - pathology; Schizophrenia - physiopathology; Statistics as Topic; Statistics, Nonparametric; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; Tyrosine 3-Monooxygenase - genetics; Tyrosine 3-Monooxygenase - metabolism; Young Adult
• ISSN: 0165-1781
• DOI: 10.1016/j.psychres.2008.10.024

The aim of this study is to profile the peripheral biomarkers (tyrosine hydroxylase, TH; interleukin-1beta, IL-1beta; and tumor necrosis factor-alpha, TNF-alpha) for schizophrenia and explore their relations with clinical symptoms. Thirty-nine patients with schizophrenia were evaluated using the Positive and Negative Syndrome Scale (PANSS), and 25 siblings and 30 normal healthy subjects were used as controls. The mRNA expression levels of TH, IL-1beta and TNF-alpha in peripheral blood mononuclear cells, as determined with semi-quantitative reverse transcription-polymerase chain reaction, were all significantly increased in both patients (3-fold) and siblings (2-fold) as compared with normal control. Both IL-1beta and TNF-alpha were significantly correlated with scores on the general psychopathology subscale of the PANSS. A significant positive correlation between IL-1beta and TH expression was found in both sibling and normal controls, but not in patients, while a positive correlation between IL-1beta and TNF-alpha was significant in all the groups. These results suggest that TH, IL-1beta and TNF-alpha are overexpressed in the peripheral blood mononuclear cells of schizophrenia patients, perhaps due to the hereditary factors. IL-1beta and TNF-alpha may influence the symptoms of schizophrenia in the cognition dysfunction and anxiety/depression domains of the PANSS.