Feeding purified glycerol from biodiesel to CBA/CA mice: effects on Gadd45a and Nfkappab1 expressions

• Published in: In vivo (Athens) Vol. 24; no. 3; pp. 303 - 307
• Main Authors: Szele, Eszter, Gombos, Katalin, Kovács, András, Ember, István
• Format: Journal Article
• Language: English
• Published: Greece 01.05.2010
• Subjects: Animal Feed; Animals; Biofuels; Biological Assay; Bone Marrow - drug effects; Bone Marrow - physiology; Carcinogens; Cell Cycle Proteins - genetics; Female; Gene Expression Regulation, Neoplastic - drug effects; Glycerol - pharmacology; Liver - drug effects; Liver - physiology; Male; Mice; Mice, Inbred CBA; NF-kappa B - genetics; Nuclear Proteins - genetics; Up-Regulation - drug effects
• ISSN: 0258-851X

The turn towards renewable energy sources has increased the production of biodiesel from rapeseed oil, leaving glycerol as a valuable by-product. Several studies have evaluated this product in feed for poultry, swine and ruminants. We investigated the effect of these glycerol products on the expression of DNA damage-inducible genes in mice. CBA/CA mice were administered two different purified glycerol products (SZME2, SZME3) for 3, 6 and 24 hours. After dietary exposure, gene expressions of nuclear factor kappa-light-chain-enhancer of activated B-cells 1 (NFkb1) and growth arrest and DNA-damage-inducible protein 45 alpha (Gadd45a) were analysed. SZME2 induced a down-regulation of the two genes in all investigated organs, including the bone marrow in both genders. After administration of SZME3, up-regulation of the two genes was observed in bone marrow in males, and the up-regulation of Gadd45a in liver, also in males. Based upon our data, SZME3, which contains glycerol of higher purification, did not induce down-regulation in genes which are involved in apoptosis.