CD14++CD16- and CD14+CD16+ human monocytes adhesion to endothelial cells
Two subsets of monocytes were identified in humans and other mammals blood based on different levels of CD14 and CD16 expression. These subsets have different patterns of adhesion molecules and chemokine receptors, which suggest different modes of interaction with endothelium and tissue traffic. Her...
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Published in | T͡S︡itologii͡a Vol. 52; no. 5; p. 380 |
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Main Authors | , , |
Format | Journal Article |
Language | Russian |
Published |
Russia (Federation)
2010
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Subjects | |
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Abstract | Two subsets of monocytes were identified in humans and other mammals blood based on different levels of CD14 and CD16 expression. These subsets have different patterns of adhesion molecules and chemokine receptors, which suggest different modes of interaction with endothelium and tissue traffic. Here, we investigated the ability of CD14+CD16+ and CD14++CD16- monocytes to adhesion to endothelial cells monolayer in presence and in the absence of pro- and anti-inflammatory cytokines. We demonstrated that CD14+CD16+ monocytes had higher level of adhesion to intact endothelial cells monolayer than CD14++CD16- monocytes. Significant increase in adhesion of CD14++CD16- and CD14+CD16+ monocytes subpopulations was observed in the presence of both TNF alpha and TNF alpha combinations with other cytokines. IFN gamma and IL-4 showed no independent effects on adhesion of monocytes. These results have demonstrated that both CD14++CD16- and CD14+CD16+ monocytes can be recruited to inflamed endothelium, but, in the absence of inflammation, CD14+CD16+ monocytes adhere to endothelial cells two times stronger than CD14++CD16- monocytes. |
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AbstractList | Two subsets of monocytes were identified in humans and other mammals blood based on different levels of CD14 and CD16 expression. These subsets have different patterns of adhesion molecules and chemokine receptors, which suggest different modes of interaction with endothelium and tissue traffic. Here, we investigated the ability of CD14+CD16+ and CD14++CD16- monocytes to adhesion to endothelial cells monolayer in presence and in the absence of pro- and anti-inflammatory cytokines. We demonstrated that CD14+CD16+ monocytes had higher level of adhesion to intact endothelial cells monolayer than CD14++CD16- monocytes. Significant increase in adhesion of CD14++CD16- and CD14+CD16+ monocytes subpopulations was observed in the presence of both TNF alpha and TNF alpha combinations with other cytokines. IFN gamma and IL-4 showed no independent effects on adhesion of monocytes. These results have demonstrated that both CD14++CD16- and CD14+CD16+ monocytes can be recruited to inflamed endothelium, but, in the absence of inflammation, CD14+CD16+ monocytes adhere to endothelial cells two times stronger than CD14++CD16- monocytes. |
Author | Freĭdlin, I S Starikova, E A Lebedeva, A M |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/20586272$$D View this record in MEDLINE/PubMed |
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SubjectTerms | Cell Adhesion - drug effects Cells, Cultured Endothelial Cells - physiology Humans Interleukin-4 - pharmacology Lipopolysaccharide Receptors - immunology Monocytes - drug effects Monocytes - immunology Monocytes - physiology Receptors, IgG - immunology Tumor Necrosis Factor-alpha - pharmacology |
Title | CD14++CD16- and CD14+CD16+ human monocytes adhesion to endothelial cells |
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