CD14++CD16- and CD14+CD16+ human monocytes adhesion to endothelial cells

Two subsets of monocytes were identified in humans and other mammals blood based on different levels of CD14 and CD16 expression. These subsets have different patterns of adhesion molecules and chemokine receptors, which suggest different modes of interaction with endothelium and tissue traffic. Her...

Full description

Saved in:
Bibliographic Details
Published inT͡S︡itologii͡a Vol. 52; no. 5; p. 380
Main Authors Starikova, E A, Lebedeva, A M, Freĭdlin, I S
Format Journal Article
LanguageRussian
Published Russia (Federation) 2010
Subjects
Cell Adhesion - drug effects
Cells, Cultured
Endothelial Cells - physiology
Humans
Interleukin-4 - pharmacology
Lipopolysaccharide Receptors - immunology
Monocytes - drug effects
Monocytes - immunology
Monocytes - physiology
Receptors, IgG - immunology
Tumor Necrosis Factor-alpha - pharmacology
Online AccessGet more information

Cover

More Information
Summary:Two subsets of monocytes were identified in humans and other mammals blood based on different levels of CD14 and CD16 expression. These subsets have different patterns of adhesion molecules and chemokine receptors, which suggest different modes of interaction with endothelium and tissue traffic. Here, we investigated the ability of CD14+CD16+ and CD14++CD16- monocytes to adhesion to endothelial cells monolayer in presence and in the absence of pro- and anti-inflammatory cytokines. We demonstrated that CD14+CD16+ monocytes had higher level of adhesion to intact endothelial cells monolayer than CD14++CD16- monocytes. Significant increase in adhesion of CD14++CD16- and CD14+CD16+ monocytes subpopulations was observed in the presence of both TNF alpha and TNF alpha combinations with other cytokines. IFN gamma and IL-4 showed no independent effects on adhesion of monocytes. These results have demonstrated that both CD14++CD16- and CD14+CD16+ monocytes can be recruited to inflamed endothelium, but, in the absence of inflammation, CD14+CD16+ monocytes adhere to endothelial cells two times stronger than CD14++CD16- monocytes.
ISSN:0041-3771