Resonance scattering detection of trace Hg2+ using herring sperm DNA modified nanogold

In pH 7.0 tris-HCl buffer solutions and in the presence of 0.017 mol x L(-1) NaCl, herring sperm DNA was combined with gold nanoparticles in size of 10 nm to form stable complex, and the NaCl did not cause the aggregation of the gold nanoparticles. Upon addition of Hg2+, that reacted with DNA to for...

Full description

Saved in:
Bibliographic Details
Published inGuang pu xue yu guang pu fen xi Vol. 30; no. 2; p. 486
Main Authors Ling, Shao-Ming, Li, Jian-Fu, Liang, Ai-Hui, Wen, Gui-Qing, Kang, Cai-Yan, Jiang, Zhi-Liang
Format Journal Article
LanguageChinese
Published China 01.02.2010
Subjects
Animals
DNA
Fishes
Gold
Limit of Detection
Male
Mercury - analysis
Metal Nanoparticles
Seafood
Spermatozoa
Online AccessGet more information

Cover

More Information
Summary:In pH 7.0 tris-HCl buffer solutions and in the presence of 0.017 mol x L(-1) NaCl, herring sperm DNA was combined with gold nanoparticles in size of 10 nm to form stable complex, and the NaCl did not cause the aggregation of the gold nanoparticles. Upon addition of Hg2+, that reacted with DNA to form more stable complex of Hg(2+)-DNA, and the gold nanoparticles aggregated to from larger nanogold clusters that led to considerable enhancement of the resonance scattering intensity at 572 nm enhanced considerably. The effect of GN concentration, DNA concentration, NaCl concentration, incubation time, and temperature, and ultrasonic irradiation was considered respectively, the conditions of 3.87 microg x mL(-1) GN, 11.7 microg x mL(-1) DNA, pH 7.0 Tris-HCl buffer solutions, 17 mmol x L(-1) NaCl, and incubation 10 min at 37 degrees C under the ultrasonic irradiation were chosen for use. Under the conditions, the enhanced resonance scattering intensity at 572 nm was linear to the Hg2+ concentration in the range of 3
ISSN:1000-0593