Affinity ligands from chemical combinatorial libraries

The above discussion provides examples of how to utilize the possibilities arising from different scenarios, related to the level of information available, to identify low molecular weight organic molecule affinity ligands to target proteins. In Table 10.1 the different published results are summari...

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Bibliographic Details
Published inMethods of biochemical analysis Vol. 54; p. 259
Main Authors Carredano, Enrique, Baumann, Herbert
Format Journal Article
LanguageEnglish
Published United States 2011
Subjects
Animals
Binding, Competitive
Combinatorial Chemistry Techniques - methods
Humans
Ligands
Molecular Structure
Organic Chemicals - chemistry
Organic Chemicals - isolation & purification
Organic Chemicals - metabolism
Protein Binding
Proteins - metabolism
Small Molecule Libraries
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Summary:The above discussion provides examples of how to utilize the possibilities arising from different scenarios, related to the level of information available, to identify low molecular weight organic molecule affinity ligands to target proteins. In Table 10.1 the different published results are summarized in terms of the structure of the ligand, the target protein, and a reference to the relevant publication. Common to all reported cases of small molecule affinity ligands is a considerably lower selectivity and affinity compared to natural protein ligands. This lower affinity has to be compensated with more thorough work in the optimization of binding and elution conditions to obtain significant recoveries and purification factors.
ISSN:0076-6941