Quantification of chloride channel 2 (CLCN2) gene isoforms in normal versus lesion- and epilepsy-associated brain tissue

The chloride channel 2 (CLCN2) gene codes for a protein organized in N- and C-terminal regions with regulatory functions and a transmembrane region which forms the ring of the pore. Mutations in the gene have previously been described in patients with idiopathic familial epilepsy. In this study we l...

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Published inBiochimica et biophysica acta Vol. 1772; no. 1; pp. 15 - 20
Main Authors Bertelli, Matteo, Cecchin, Stefano, Lapucci, Cristina, de Gemmis, Paola, Danieli, Daniela, d'Amore, Emanuele S G, Buttolo, Luciano, Giunta, Filippo, Mortini, Pietro, Pandolfo, Massimo
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.2007
Subjects
Aged
Aged, 80 and over
Alternative Splicing
Brain - metabolism
Brain - pathology
Chloride Channels - biosynthesis
Chloride Channels - genetics
Epilepsy - genetics
Epilepsy - metabolism
Epilepsy - pathology
Female
Gene Expression
Humans
Male
Middle Aged
Polymerase Chain Reaction
Protein Isoforms - biosynthesis
Protein Isoforms - genetics
RNA, Messenger - analysis
RNA, Messenger - genetics
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Summary:The chloride channel 2 (CLCN2) gene codes for a protein organized in N- and C-terminal regions with regulatory functions and a transmembrane region which forms the ring of the pore. Mutations in the gene have previously been described in patients with idiopathic familial epilepsy. In this study we looked for new isoforms of CLCN2 and we estimated expression levels by real time PCR in brain tissue containing epileptic foci. Samples used in this study were first analyzed and selected to exclude mutations in the coding region of the gene. Four isoforms (skipping exons 3, 16, 22 and 6/7) were identified and quantified by Real Time PCR and compared with total expression of the gene. Expression of the region common to all CLCN2 isoforms was 50% less in epilepsy-associated brain tissue than in controls. The ratio of the various isoforms was slightly greater in epileptic than control tissue. The greatest difference was recorded in the temporal lobe for the isoform with skipped exon 22. Analysis of these isoforms in brain tissue containing epileptic foci suggests that CLCN2 could be implicated in epilepsy, even in the absence of mutations.
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ISSN:0006-3002