Effect of adminitration of etidronate and retinol on bone mechanical properties in ovariectomized rats

• Published in: Pharmacological reports Vol. 57; no. 2; p. 203
• Main Authors: Kaczmarczyk-Sedlak, Ilona, Janiec, Waldemar, Pytlik, Maria, Sliwiński, Leszek, Folwarczna, Joanna, Cegieła, Urszula, Nowińska, Barbara, Barnaś, Małgorzata
• Format: Journal Article
• Language: English
• Published: Switzerland 01.03.2005
• Subjects: Animals; Biomechanical Phenomena; Body Weight - drug effects; Bone and Bones - drug effects; Bone and Bones - metabolism; Bone Density - drug effects; Bone Density Conservation Agents - administration & dosage; Bone Density Conservation Agents - pharmacology; Bone Remodeling - drug effects; Bone Remodeling - physiology; Calcification, Physiologic - drug effects; Drug Interactions; Etidronic Acid - administration & dosage; Etidronic Acid - pharmacology; Female; Femur - drug effects; Femur - metabolism; Lumbar Vertebrae - drug effects; Lumbar Vertebrae - metabolism; Organ Size - drug effects; Ovariectomy; Rats; Rats, Wistar; Tibia - drug effects; Tibia - metabolism; Vitamin A - administration & dosage; Vitamin A - pharmacology
• ISSN: 1734-1140

The experiments were carried out on rats, divided into 7 groups: I-sham-operated control rats, II-ovariectomized (OVX) control rats, II- OVX+etidronate (10 mg/kg po), IV-OVX+retinol (700 IU/kg po), V-OVX+retinol (3,500 IU/kg po), VI-OVX+etidronate (10 mg/kg po)+retinol (700 IU/kg po), VII-OVX+etidronate (10 mg/kg po)+retinol (3 500 IU/kg po). The drugs were administered once a day for 4 weeks. Bone mass, content of mineral substances and calcium were examined in the femur, tibia and L-4 vertebra. In the femur, mechanical properties of the whole bone supported on its epiphyses (the ultimate load, the breaking load and the deformation caused by the applied load) and of the femoral neck (the load causing the fracture) were studied. Bilateral ovariectomy induced unfavorable changes in mechanical properties of rat bones, which were partially prevented by administration of etidronate. Retinol at 3,500 IU/kg po caused intensification of the osteoporotic changes, especially it worsened the mechanical properties of bones. The results of the present study do not indicate the existence of any interaction between retinol and etidronate concerning mechanical properties of bones in OVX rats.