Basic principles of the antisense strategy

The antisens strategy is based on specific inhibition of the mutant gene expression by oligodeoxynucleotides (OGN), capable of selectively hybridizing with target DNA or RNA. Molecular mechanisms of the antisense oligonucleotide comprise: inhibition of splicing, inhibition of 5'-capping and 3&#...

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Bibliographic Details
Published inRevista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi Vol. 107; no. 3; p. 483
Main Authors Tauşer, Roxana Georgiana, Stoica, Ortansa
Format Journal Article
LanguageRomanian
Published Romania 01.07.2003
Subjects
Enzyme Activation
Gene Expression Regulation
Humans
Mutation
Oligodeoxyribonucleotides, Antisense - genetics
Polyadenylation
Ribonuclease H - metabolism
RNA Splicing - genetics
RNA, Catalytic - metabolism
RNA, Ribosomal - genetics
RNA, Small Interfering - metabolism
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Summary:The antisens strategy is based on specific inhibition of the mutant gene expression by oligodeoxynucleotides (OGN), capable of selectively hybridizing with target DNA or RNA. Molecular mechanisms of the antisense oligonucleotide comprise: inhibition of splicing, inhibition of 5'-capping and 3'-polyadenylation, activation of RN-ase H, small interfering RNA, ribozymes. The antisense strategy is applied to: rational design of potent, selective therapeutic agents, target validation and detection of pathologic gene expression in vivo. The main aim of the antisense oligonucleotide design is to improve the affinity for target RNA and the resistance to nucleolytic degradation.
ISSN:0048-7848