alpha1-acid glycoprotein possesses in vitro pro- and antiinflammatory activities

• Published in: Bulletin of experimental biology and medicine Vol. 131; no. 5; pp. 479 - 481
• Main Authors: Pukhal'skii, A L, Shmarina, G V, Lyutov, A G, Novikova, L I, Shemyakin, I G, Aleshkin, V A
• Format: Journal Article
• Language: English
• Published: United States 01.05.2001
• Subjects: Cell Division; Cells, Cultured; Concanavalin A - chemistry; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Humans; Inflammation - metabolism; Interleukin-10 - biosynthesis; Leukocytes - metabolism; Models, Biological; Orosomucoid - metabolism; Phytohemagglutinins - metabolism; Sepharose - chemistry; Tumor Necrosis Factor-alpha - biosynthesis; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0007-4888

We studied the effects of alpha1-acid glycoprotein on tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) production and lymphocyte response to phytohemagglutinin in cultured peripheral blood mononuclear leukocytes from 6 healthy donors. We observed 2 opposite responses to alpha1-acid glycoprotein: first, stimulation of TNF-alpha and IL-10 production and inhibition of lymphocyte proliferation, and second, suppression of cytokine production and stimulation of lymphocyte proliferation. In cell cultures isolated from 4 of 6 donors, the TNF-alpha/IL-10 ratio remained unchanged after addition of native alpha1-acid glycoprotein, but some fractions isolated by chromatography on concanavalin A-Sepharose changed this parameter. These changes were most pronounced after treatment with fraction C enriched with molecules with incomplete (biantennary) carbohydrate chains. The mechanisms of alpha1-acid glycoprotein-induced effects on peripheral blood mononuclear leukocytes are discussed.