Radioenzymatic investigation of membrane and soluble forms of angiotensin-converting enzyme in acetate-phosphate buffer

The dose response of soluble and membrane forms of angiotensin-converting enzyme to gamma-irradiation is investigated at different pH values of the medium and at different concentrations of acetate-phosphate buffer. Membrane form of the enzyme is more stable shows principally other conformational eq...

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Bibliographic Details
Published inRadiat͡s︡ionnai͡a︡ biologii͡a︡, radioėkologii͡a Vol. 43; no. 4; p. 452
Main Authors Orlova, M A, Kost, O A, Grinshteĭn, S V, Fedoseev, V M, Korzhuev, A V, Troshina, N N
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.2003
Subjects
Acetates
Buffers
Dose-Response Relationship, Radiation
Enzyme Stability - radiation effects
Gamma Rays
Hydrogen-Ion Concentration
Membranes - enzymology
Membranes - radiation effects
Peptidyl-Dipeptidase A - chemistry
Peptidyl-Dipeptidase A - radiation effects
Phosphates
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Summary:The dose response of soluble and membrane forms of angiotensin-converting enzyme to gamma-irradiation is investigated at different pH values of the medium and at different concentrations of acetate-phosphate buffer. Membrane form of the enzyme is more stable shows principally other conformational equilibrium than the soluble form. "Splitted" activation peaks on the curves of the enzyme dose response are observed.
ISSN:0869-8031