Further development (MDCK) of live cold-adapted influenza vaccine: cultivation of vaccine strains in production fermenters

Optimal conditions were developed for cultivating the cold-adapted reassortant live influenza vaccine (CARLIV) in MDCK cells, which were in their turn cultivated in fermenters with serum-free medium and microcarrier. The use of MDCK cells meets all national and WHO requirements to continuous cells u...

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Bibliographic Details
Published inVoprosy virusologiĭ Vol. 50; no. 2; p. 4
Main Authors Gendon, Iu Z, Markushin, S G, Akopova, I I, Koptiaeva, I B, Nechaeva, E A, Mazurkova, I A, Radaeva, I F, Kolokol'tsova, T D
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.2005
Subjects
Animals
Bioreactors
Cell Line
Cold Temperature
Culture Media, Serum-Free
Dogs
Influenza A virus - genetics
Influenza A virus - growth & development
Influenza Vaccines - genetics
Influenza Vaccines - standards
Mutation
Reassortant Viruses - genetics
Reassortant Viruses - growth & development
Virus Cultivation
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Summary:Optimal conditions were developed for cultivating the cold-adapted reassortant live influenza vaccine (CARLIV) in MDCK cells, which were in their turn cultivated in fermenters with serum-free medium and microcarrier. The use of MDCK cells meets all national and WHO requirements to continuous cells used in the production of biological preparations. CARLIV cultivated under such conditions well preserve their ts-mutations and mutation, which entail substitutions of amino acids, in all CARLIV genome segments. Provided the cultivation conditions are optimal, the output of multivalent CARLIV in a 101 fermenter can reach 100000 doses.
ISSN:0507-4088