The effect of sinomenine on cyclooxygenase activity and the expression of COX-1 and COX-2 mRNA in human peripheral monocytes

• Published in: Zhongguo zhongyao zazhi Vol. 28; no. 4; p. 352
• Main Authors: Wang, Wen-jun, Wang, Pei-xun, Li, Xiao-juan
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2003
• Subjects: Adult; Cyclooxygenase 1; Cyclooxygenase 2; Dinoprostone - blood; Humans; Isoenzymes - biosynthesis; Leukocytes, Mononuclear - enzymology; Membrane Proteins; Morphinans - isolation & purification; Morphinans - pharmacology; Plants, Medicinal - chemistry; Prostaglandin-Endoperoxide Synthases - biosynthesis; Prostaglandin-Endoperoxide Synthases - metabolism; RNA, Messenger - biosynthesis; Sinomenium - chemistry
• ISSN: 1001-5302

To observe in vitro the effect of Sinomenine, a pure alkaloid extracted from the chinese medical plant Sinomenium acutum on the activity of cyclooxygenase (COX-1 and COX-2) and the expression of COX-1 and COX-2 mRNA. Mononuclear leukocytes were obtained from healthy adults. Isolated mononuclear leucocytes from human peripheral blood (PBMC) were incubated (1 x 10(6).mL-1) with or without sinomenine (or indomethacin), after incubated for 24 hours at 37 degrees C with 5% CO2; the media were assayed for the PGE2 by radioimmunoassay (RIA). LPS was used to stimulate the monocytes at a concentration of 5 micrograms.mL-1. And by RT-PCR, both COX-1 and COX-2 mRNAs were detected in Mononuclear leukocytes after incubation for different hours with drug (sinomenine or indomethacin) or not. LPS (stimulated) induced the production of PGE2 in PBMC increasing with high expression of COX-2 mRNA; sinomenine reduced PGE2 production in LPS stimulated human monocytes more than in non-stimulated human monocytes. In comparative expe