Comparative assessment of determination of measles virus IgG antibodies in blood serum and dried whole blood

Enzyme immunoassay (Dade Behring Enzygnost diagnostic kit, Germany) was used to estimate the levels of measles virus IgG antibodies in 72 samples of blood serum and eluates of the whole blood dried on the Whatman 3M filter paper. The sensitivity of the dried blood test was 98.4%. The regression anal...

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Bibliographic Details
Published inVoprosy virusologiĭ Vol. 53; no. 1; p. 46
Main Authors Samoĭlovich, E O, Svirichevskaia, E Iu, Ermolaev, M A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.2008
Subjects
Adolescent
Adult
Aged
Antibodies, Viral - analysis
Blood Specimen Collection - methods
Fluorescent Antibody Technique, Direct
Humans
Immunoenzyme Techniques
Immunoglobulin G - analysis
Measles - blood
Measles virus - immunology
Middle Aged
Sensitivity and Specificity
Serologic Tests
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Summary:Enzyme immunoassay (Dade Behring Enzygnost diagnostic kit, Germany) was used to estimate the levels of measles virus IgG antibodies in 72 samples of blood serum and eluates of the whole blood dried on the Whatman 3M filter paper. The sensitivity of the dried blood test was 98.4%. The regression analysis showed a high correlation of the results (r2 = 0.89; regression coefficient 0.89). Measles virus IgG antibodies were ascertained to be retained in the dried blood at room temperature long (for at least 2 years). After 2-year storage at +4 degrees C, there was a reduction in the concentration of antibodies; however, they were still detectable in 62 (96.9%) out of 64 positive samples. The dried blood test is recommended for wide use to study immunity to measles virus.
ISSN:0507-4088