Functional activity of lymphoblastoid cells infected by human adenovirus type 2 and Epstein-Barr virus

• Published in: Mikrobiolohichnyĭ zhurnal (Kiev, Ukraine : 1993) Vol. 67; no. 3; p. 71
• Main Authors: Povnitsa, O Iu, Diachenko, N S, Nosach, L N, Olevinskaia, Z M, Zhovnovataia, V L, Polishchuk, V N, Spivak, N Ia
• Format: Journal Article
• Language: Russian
• Published: Ukraine 01.05.2005
• Subjects: Adenovirus Infections, Human - immunology; Adenovirus Infections, Human - virology; B-Lymphocytes - immunology; B-Lymphocytes - virology; Cells, Cultured; Epithelial Cells - immunology; Epithelial Cells - virology; Hemagglutinins; Herpesviridae Infections - immunology; Herpesviridae Infections - virology; Interferon Inducers; Interferon-alpha - biosynthesis; Interferon-gamma - biosynthesis; Newcastle disease virus; T-Lymphocytes - immunology; T-Lymphocytes - virology; Tumor Necrosis Factor-alpha - biosynthesis
• ISSN: 1028-0987

The paper deals with the influence of the adenovirus (Ad) and Epstein-Barr virus (EBV) on functional activity of lymphocytes, in particular, the production of alpha- and gamma-interferons, tumor necrosis factor (TNF) in conditions of mono- or double infection of B- and T-phenotype (CEM) lymphoblastoid cells. It is shown, that Ad, EBV or both viruses induce high enough levels of interferon on both lines of cells and in control epithelial cells. The lymphoblastoid cells infected by viruses deep ability to synthesize alpha- and gamma-interferons under the influence of the corresponding inducers (Newcastle disease virus and hemagglutinine). Nevertheless, the levels of their formation are not high. Rather high parameters of activity of the tumor necrosis factor (TNF) were revealed during a day in the initial B95-8 cells and superinfected Ad after the effect of LPS of E. coli. Their activity in CEM cells also did not depend on the infection type.