Evaluation of semi-quantitative PCR and IgG & IgM ELISA in diagnosis of toxoplasmosis in females with miscarriage

One hundred female (age 20-42 yrs) patients were classified into group I: 40 patients presented with abortion in the first trimester; group II: 33 patients with abortion in the second trimester and group III: 27 patients with intrauterine fetal death (IUFD). The positive percentages of semi-quantita...

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Bibliographic Details
Published inJournal of the Egyptian Society of Parasitology Vol. 34; no. 2; p. 559
Main Authors Abdel-Hameed, Dina M, Hassanein, Omayma
Format Journal Article
LanguageEnglish
Published Egypt 01.08.2004
Subjects
Abortion, Spontaneous - parasitology
Animals
Antibodies, Protozoan - blood
DNA, Protozoan - chemistry
DNA, Protozoan - genetics
Enzyme-Linked Immunosorbent Assay
False Negative Reactions
Female
Humans
Immunoglobulin G - blood
Immunoglobulin M - blood
Polymerase Chain Reaction - methods
Pregnancy
Pregnancy Complications, Parasitic - diagnosis
Sensitivity and Specificity
Toxoplasma - genetics
Toxoplasma - growth & development
Toxoplasmosis - complications
Toxoplasmosis - diagnosis
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Summary:One hundred female (age 20-42 yrs) patients were classified into group I: 40 patients presented with abortion in the first trimester; group II: 33 patients with abortion in the second trimester and group III: 27 patients with intrauterine fetal death (IUFD). The positive percentages of semi-quantitative PCR and both IgG & IgM ELISA were 38% and 35% respectively. Ten (26.3%) cases out of 38 were positive for toxoplasmosis by both PCR and ELISA-IgG, while 5 (13.2%) cases out of 38 were positive by both PCR and ELISA-IgM, whereas 16 (42.1%) cases out of 38 PCR positive cases were positive by both ELISA IgG & IgM. Sensitivity and specificity of both ELISA IgG and IgM were 81.57% & 93.54% respectively. False negative by ELISA were found in 7 cases out of 38 positive toxoplasmosis cases detected by semi-quantitative PCR. Three cases out of the 7 cases with false negative by ELISA were detected with a trophozoite copy load of 10(1) trophozoite /mL in the blood sample by semi-quantitative PCR. So, the semi-quantitative PCR detected low levels of parasite DNA recommending its usefulness especially in the early stages of the disease when low amount of antibodies can't be detected by serological method or even by the conventional PCR.
ISSN:1110-0583