Physical interaction between hepatitis C virus NS4B protein and CREB-RP/ATF6beta

By using a yeast two-hybrid assay, cyclic AMP-response-element-binding protein-related protein (CREB-RP), also called activating transcription factor 6beta (ATF6beta), was identified as a cellular protein that interacts with the NS4B protein of hepatitis C virus. An N-terminal half of NS4B and a cen...

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Published inBiochemical and biophysical research communications Vol. 299; no. 3; pp. 366 - 372
Main Authors Tong, Wen Yan, Nagano-Fujii, Motoko, Hidajat, Rachmat, Deng, Lin, Takigawa, Yuki, Hotta, Hak
Format Journal Article
LanguageEnglish
Published United States 06.12.2002
Subjects
Activating Transcription Factor 6
Amino Acid Sequence
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - metabolism
Fungal Proteins - genetics
Fungal Proteins - metabolism
HeLa Cells
Humans
Leucine Zippers
Macromolecular Substances
Microscopy, Fluorescence
Molecular Sequence Data
Protein Binding
Protein Structure, Tertiary
Transcription Factors - chemistry
Transcription Factors - metabolism
Two-Hybrid System Techniques
Viral Nonstructural Proteins - genetics
Viral Nonstructural Proteins - metabolism
Yeasts - genetics
Yeasts - metabolism
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Summary:By using a yeast two-hybrid assay, cyclic AMP-response-element-binding protein-related protein (CREB-RP), also called activating transcription factor 6beta (ATF6beta), was identified as a cellular protein that interacts with the NS4B protein of hepatitis C virus. An N-terminal half of NS4B and a central portion of CREB-RP/ATF6beta containing the basic leucine zipper (bZIP) domain were involved in the interaction. The interaction between NS4B and CREB-RP/ATF6beta was demonstrated also in mammalian cells by co-immunoprecipitation and confocal microscopic analyses using specific antibodies. The bZIP domain of ATF6alpha, which shares high sequence similarity with CREB-RP/ATF6beta, was also shown to interact with NS4B in yeast although the interaction was weaker than that between NS4B and CREB-RP/ATF6beta. CREB-RP/ATF6beta and ATF6alpha are known as endoplasmic reticulum (ER) stress-induced transcription factors. Collectively, our results imply the possibility that NS4B modulates certain cellular responses upon ER stress through the physical interaction with CREB-RP/ATF6beta and ATF6alpha.
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ISSN:0006-291X