Study on the anti-apopotosis induced by hypoxia/hypoglycemia and reoxygenation of panax notoginseng saponins in cultured rat hippocampal neurons

To study the inhibitory effects of Panax Notoginseng Saponins(PNS) on apoptosis induced by hypoxia/hypoglycemia and reoxygenation in cultured rat hippocampal neurons. Apoptosis were measured by flow cytometry, intracellular free calcium concentration([Ca2+]i) was measured with confocal laser scannin...

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Published inZhongguo zhongyao zazhi Vol. 28; no. 1; p. 52
Main Authors Zhu, Ling-Qun, Fan, Ji-ping, Huang, Qi-fu, Sun, Su-lun, Gao, Ying, Zou, Yi-huai, Zhang, Zhuang, He, Li-yun, Zheng, Hong
Format Journal Article
LanguageChinese
Published China 01.01.2003
Subjects
Animals
Apoptosis - drug effects
Calcium - metabolism
Cell Hypoxia
Cells, Cultured
Fetus
Ginsenosides - isolation & purification
Ginsenosides - pharmacology
Hippocampus - cytology
Hypoglycemia - pathology
L-Lactate Dehydrogenase - metabolism
Neurons - cytology
Panax - chemistry
Plants, Medicinal - chemistry
Rats
Rats, Wistar
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Summary:To study the inhibitory effects of Panax Notoginseng Saponins(PNS) on apoptosis induced by hypoxia/hypoglycemia and reoxygenation in cultured rat hippocampal neurons. Apoptosis were measured by flow cytometry, intracellular free calcium concentration([Ca2+]i) was measured with confocal laser scanning microscopy, morphological changes and neuronal necrosis were observed with fluorescence microscope, and meanwhile the leakage of lactic dehydrogenase(LDH) was measured. Hypoxia/hypoglycemia cultures for 5 hours and reoxygenation induced neuronal apoptosis and necrosis, and significantly increased neuronal [Ca2+]i and the leakage of LDH. The effects were increased with the extending time of reoxygenation. PNS has could significantly decrease the percentage of neuronal apoptosis and necrosis, and reduce neuronal [Ca2+]i and the leakage of LDH. PNS has inhibitory effect on neuronal apoptosis. This effect might be related to its effect of decreasing intracellular free calcium concentration.
ISSN:1001-5302