Degradation of the herbicide atrazine by the soil mycelial fungus INBI 2-26(-)--a producer of cellobiose dehydrogenase

• Published in: Prikladnaja biohimija i mikrobiologija Vol. 40; no. 3; p. 337
• Main Authors: Khromonygina, V V, Saltykova, A I, Vasil'chenko, L G, Kozlov, Iu P, Rabinovich, M L
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.05.2004
• Subjects: Atrazine - metabolism; beta-Glucosidase - analysis; beta-Glucosidase - biosynthesis; Biodegradation, Environmental; Carbohydrate Dehydrogenases - analysis; Carbohydrate Dehydrogenases - biosynthesis; Carbohydrate Dehydrogenases - metabolism; Cellulases - analysis; Cellulases - biosynthesis; Culture Media; Dioxins - analysis; Fungi - chemistry; Fungi - growth & development; Fungi - metabolism; Herbicides - metabolism; Mycelium; Soil Microbiology; Time Factors; Vietnam; Vietnam
• ISSN: 0555-1099

Nonsporulating mycelial fungi producing cellobiose dehydrogenase (CDH) and isolated from soils of South Vietnam with high residual content of dioxins are capable of growing on a solid medium in the presence of high atrazine concentrations (to 500 mg/l). At 20 and 50 mg/l atrazine, the area of fungal colonies was 1.5-1.2-fold larger, respectively, compared with control colonies of the same age, whereas development of the colonies at 500 mg/l atrazine was delayed by 5 days, compared with controls grown in the absence of atrazine. Surface cultivation of the fungus on a minimal medium with glucose as a sole source of carbon and energy decreased the initial concentration of atrazine (20 mg/l) 50 times in 40 days; in addition, no pronounced sorption of atrazine by mycelium was detected. This was paralleled by accumulation in the culture medium of extracellular CDH; atrazine increased the synthesis of this enzyme two- to threefold. Accumulation of beta-glucosidase (a mycelium-associated enzyme) and cellulases preceded the formation of CDH.