4-Hydroxybenzoate uptake in Klebsiella planticola strain DSZ1 is driven by DeltapH

Klebsiella planticola strain DSZ1 has the ability to degrade different aromatic compounds such as benzoate and organochlorinated as propachlor and alachlor. DSZ1 strain cells mineralised 4-hydroxybenzoate (4HBA) through a meta-cleavage pathway, yielding protocatechuate as dihydroxylated intermediate...

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Published inCurrent microbiology Vol. 44; no. 1; p. 31
Main Authors Allende, J L, Gibello, A, Fortún, A, Sánchez, M, Martín, M
Format Journal Article
LanguageEnglish
Published United States 01.01.2002
Subjects
Adenosine Triphosphate - analysis
Adenosine Triphosphate - metabolism
Anaerobiosis
Animals
Benzoates - metabolism
Benzoates - pharmacology
Biological Transport - drug effects
Hydrogen-Ion Concentration
Klebsiella - metabolism
Membrane Potentials - drug effects
Parabens - metabolism
Sodium Azide - metabolism
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Summary:Klebsiella planticola strain DSZ1 has the ability to degrade different aromatic compounds such as benzoate and organochlorinated as propachlor and alachlor. DSZ1 strain cells mineralised 4-hydroxybenzoate (4HBA) through a meta-cleavage pathway, yielding protocatechuate as dihydroxylated intermediate, with a specific rate of CO2 formation 0.12 x 10-6 (cpm/OD) h-1, and a rate of 4-HBA utilisation of 0.75 mmol h-1. Aerobically the 4HBA transport system is driven by gradient of protons (DeltapH), but is not ATP-driven. Under anaerobic conditions, the system can use the nitrate reduction as a final electron acceptor in respiration. A kinetic analysis of the 4HBA transport system revealed a Kt value of 16 microM with a Vmax value of 25 nmol/min.mg at pH 7.
ISSN:0343-8651