Microbiological characterization of candidate vaccine strains of Ballum serogroup Leptospira interrogans

Two candidate vaccines of Ballum serogroup Leptospira interrogans were microbiologically characterized as part of the work directed to the obtention of new antileptospirosis vaccine formulations for human use. The growth kynetics of both strains was evaluated in EMJH protein medium and in 3 protein...

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Published inRevista cubana de medicina tropical Vol. 55; no. 3; pp. 146 - 152
Main Authors Rodríguez, Andrés González, Jimenez, Yoandra Rodríguez, Santiesteban, Niurka Batista, Abreu, Yolanda Valdés, González, Marta González
Format Journal Article
LanguageSpanish
Published Cuba 01.09.2003
Subjects
Animals
Bacterial Vaccines
Cricetinae
Leptospira interrogans - immunology
Leptospira interrogans - isolation & purification
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Summary:Two candidate vaccines of Ballum serogroup Leptospira interrogans were microbiologically characterized as part of the work directed to the obtention of new antileptospirosis vaccine formulations for human use. The growth kynetics of both strains was evaluated in EMJH protein medium and in 3 protein free media. The virulence was estimated in hamsters by the calculation of the mean lethal dose. The cellular and extracellular antigenic profiles were analyzed by unidimensional SDS-PAGE and compared with those from strains of Canicola, Icterohaemorrhagiae and Pomona serogroups. The antigenic homology among heterologous groups was analyzed by western blotting with serun from hasterms vaccinated with vax-SPIRAL. The results obtained showed a fastidious growth of both strains of Ballum in the studied media, a high virulence in the animal model and a large antigenic homology with strains from other serogroups of Lepstospira prevailing in Cuba.
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ISSN:0375-0760