The detection of 20S-hydroxycholesterol in extracts of rat brains and human placenta by a gas chromatograph/mass spectrometry technique

The presence of 20(S)-hydroxycholesterol in rat brains and human placenta has been established using the gas chromatography/mass spectrometry (GC/MS) select ion monitoring (SIM) technique. Identification was ensured by three criteria: the specific retention time when the compound emerges from the ga...

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Published inThe Journal of steroid biochemistry and molecular biology Vol. 85; no. 1; pp. 57 - 61
Main Authors Lin, Ying Yong, Welch, Margaret, Lieberman, Seymour
Format Journal Article
LanguageEnglish
Published England 01.05.2003
Subjects
Animals
Brain Chemistry
Chromatography, Thin Layer
Female
Gas Chromatography-Mass Spectrometry
Humans
Hydroxycholesterols - chemistry
Hydroxycholesterols - isolation & purification
Placenta - chemistry
Rats
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Summary:The presence of 20(S)-hydroxycholesterol in rat brains and human placenta has been established using the gas chromatography/mass spectrometry (GC/MS) select ion monitoring (SIM) technique. Identification was ensured by three criteria: the specific retention time when the compound emerges from the gas chromatogram and the two m/z ions (201 and 461amu) which are characteristic of its mass spectrum. The possible role of 20(S)-hydroxycholesterol in steroid hormone biosynthesis and in other biological processes is discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0960-0760
1879-1220