Mixed lymphocyte cultures can predict TCR Vbeta repertoires of T cells infiltrating kidney transplants during acute rejection episodes

Alloreactive T cell populations can show skewing of T-cell antigen receptor (TCR) Vbeta gene usage. The aims of the experiments were to compare in vivo and in vitro T cell alloresponses against donor alloantigens for TCR Vbeta gene usage. T-cell cultures from renal biopsies taken during acute reject...

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Bibliographic Details
Published inTransplantation Vol. 80; no. 12; p. 1805
Main Authors Paraoan, Marius T, Bakran, Ali, Hammad, Abdul, Sells, Robert A, Christmas, Stephen E
Format Journal Article
LanguageEnglish
Published United States 27.12.2005
Subjects
Acute Disease
Biopsy
Cytotoxicity, Immunologic
Genes, T-Cell Receptor beta
Graft Rejection - immunology
Graft Rejection - pathology
Histocompatibility Testing
Humans
Kidney Transplantation - immunology
Kidney Transplantation - pathology
Lymphocyte Culture Test, Mixed - methods
Receptors, Antigen, T-Cell, alpha-beta - immunology
T-Lymphocytes - immunology
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Summary:Alloreactive T cell populations can show skewing of T-cell antigen receptor (TCR) Vbeta gene usage. The aims of the experiments were to compare in vivo and in vitro T cell alloresponses against donor alloantigens for TCR Vbeta gene usage. T-cell cultures from renal biopsies taken during acute rejection and pretransplant mixed lymphocyte cultures (MLC) were established from five renal transplant patients. TCR Vbeta gene usage, assessed with Vbeta family specific antibodies, showed that up to five different Vbeta families were significantly expanded. In four of five cases, there was close concordance between Vbeta families expanded from the biopsy and in MLC. T-cell clones from one renal biopsy were specific for the mismatched donor alloantigen and showed similar TCR Vbeta gene usage to the original T-cell line. The results show very similar patterns of TCR Vbeta gene usage in alloreactive T cells generated ex vivo or in vitro.
ISSN:0041-1337