Quantitative expression of the adhesion receptors VLA-4, VLA-5, L-selectin, MAC-1, and ICAM-1 on the surface of CD34+ cells

• Published in: Transfusion clinique et biologique : journal de la Société française de transfusion sanguine Vol. 8; no. 6; pp. 453 - 459
• Main Authors: Gigant, C, Latger-Cannard, V, Bensoussan, D, Feugier, P, Bordigoni, P, Stoltz, J F
• Format: Journal Article
• Language: French
• Published: France 01.12.2001
• Subjects: Antigens, CD - analysis; Antineoplastic Agents - pharmacology; Blood Cells - metabolism; Bone Marrow Cells - metabolism; CD18 Antigens - analysis; Cell Adhesion - physiology; Filgrastim; Gene Expression Regulation; Graft Survival; Granulocyte Colony-Stimulating Factor - pharmacology; Hematopoietic Stem Cell Mobilization; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells - metabolism; Humans; Immunophenotyping; Integrin alpha4; Integrin alpha4beta1; Integrin alpha5; Integrins - biosynthesis; Integrins - genetics; Intercellular Adhesion Molecule-1 - analysis; Intercellular Adhesion Molecule-1 - biosynthesis; Intercellular Adhesion Molecule-1 - genetics; L-Selectin - biosynthesis; L-Selectin - genetics; Macrophage-1 Antigen - analysis; Macrophage-1 Antigen - biosynthesis; Macrophage-1 Antigen - genetics; Models, Biological; Multiple Myeloma - therapy; Organ Specificity; Receptors, Fibronectin - biosynthesis; Receptors, Fibronectin - genetics; Receptors, Lymphocyte Homing - biosynthesis; Receptors, Lymphocyte Homing - genetics; Recombinant Proteins; Reproducibility of Results
• ISSN: 1246-7820

The aim of this work was to quantify by flow cytometry the main adhesion receptors on CD34+ cells. These cells were isolated from bone marrow (BM) or mobilized peripheral blood (PB). The proportions of CD34+/CD49d+ and CD34+/CD49e+ are weaker on PB cells, without quantitative expression variation. This phenotypic variation may induce CD34+ cells exist from BM into circulation, promoting the mobilization. The homing to the BM implicate the CD62L receptor, which expression was found more frequently and stronger on PB cells than on BM. The CD11b, CD18 and CD54 receptors are implicated in CD34+ cells adhesion to BM micro-environment. No significant variation in CD34+/CD11b+ and CD34+/CD18+ cells frequency was noted. Moreover, CD54 receptor was more frequently expressed on PB cells. Quantitative analysis revealed that CD18 was more strongly expressed on BM than on PB cells. This quantitative variation could promote progenitor adhesion by interacting with stromal cells. Finally, quantitative expression of the main receptors on CD34+ cells provides an original option for studying CD34+ cells during the mobilization, the homing or the adhesion to BM micro-environment.