Neuropeptide Y selectively potentiates alpha1-adrenoceptor-mediated contraction through Y1 receptor subtype in rat femoral artery

• Published in: Journal of cardiovascular pharmacology Vol. 42 Suppl 1; p. S33
• Main Authors: Tsurumaki, Tatsuru, Honglan, Piao, Higuchi, Hiroshi
• Format: Journal Article
• Language: English
• Published: United States 01.12.2003
• Subjects: Animals; Aorta, Thoracic - drug effects; Arginine - administration & dosage; Arginine - analogs & derivatives; Arginine - pharmacokinetics; Dose-Response Relationship, Drug; Drug Synergism; Femoral Artery - drug effects; Gene Expression; Humans; Male; Muscle Contraction - drug effects; Muscle, Smooth, Vascular - drug effects; Neuropeptide Y - administration & dosage; Neuropeptide Y - antagonists & inhibitors; Neuropeptide Y - pharmacokinetics; Phenylephrine - administration & dosage; Phenylephrine - pharmacokinetics; Rats; Rats, Wistar; Receptors, Adrenergic, alpha-1 - drug effects; Receptors, Neuropeptide Y - antagonists & inhibitors; Receptors, Neuropeptide Y - drug effects; Receptors, Neuropeptide Y - genetics; Reverse Transcriptase Polymerase Chain Reaction; Serotonin - administration & dosage; Serotonin - pharmacokinetics
• ISSN: 0160-2446

The aim of this study was to investigate the synergism between neuropeptide Y and other vasoconstrictors (phenylephrine and serotonin) and which neuropeptide Y receptor subtype is responsible for the neuropeptide Y-induced potentiation. Exogenous neuropeptide Y (10 nM) potentiated alpha1-adrenoceptor-mediated (PE-induced) contraction in rat femoral artery permissively without its direct action, but not in the thoracic aorta. In contrast, neuropeptide Y produced no change in serotonin-induced contraction in both arteries. Increasing concentrations of neuropeptide Y caused dose-dependent potentiation of the phenylephrine-induced contraction in the femoral artery. This potentiation was blocked by a selective neuropeptide Y-Y1 receptor antagonist, BIBP3226 [(R)-N2-(diphenylacetyl)-N-[4-hydroxyphenyl)methyl]-argininamide] (1 microM). Semiquantitative reverse transcriptase polymerase chain reaction showed the selective expression of neuropeptide Y-Y1 receptor mRNA in the femoral artery. These findings indicated that the neuropeptide Y-induced selective potentiation of alpha1-adrenoceptor-mediated contraction is mediated through neuropeptide Y-Y1 receptor in rat femoral artery.