IFNbeta induction by influenza A virus is mediated by RIG-I which is regulated by the viral NS1 protein

• Published in: Cellular microbiology Vol. 9; no. 4; pp. 930 - 938
• Main Authors: Opitz, Bastian, Rejaibi, Amira, Dauber, Bianca, Eckhard, Jamina, Vinzing, Maya, Schmeck, Bernd, Hippenstiel, Stefan, Suttorp, Norbert, Wolff, Thorsten
• Format: Journal Article
• Language: English
• Published: India 01.04.2007
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - physiology; Cell Line; Cell Line, Tumor; DEAD Box Protein 58; DEAD-box RNA Helicases - genetics; DEAD-box RNA Helicases - physiology; Enzyme-Linked Immunosorbent Assay; Epithelial Cells - metabolism; Epithelial Cells - pathology; Epithelial Cells - virology; Humans; Immunoblotting; Influenza A virus - genetics; Influenza A virus - growth & development; Interferon Regulatory Factor-3 - genetics; Interferon Regulatory Factor-3 - physiology; Interferon-beta - genetics; Interferon-beta - metabolism; Interferon-Induced Helicase, IFIH1; Mutation; Promoter Regions, Genetic - genetics; Receptors, Immunologic; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; Signal Transduction - genetics; Signal Transduction - physiology; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - physiology
• ISSN: 1462-5814

Influenza A virus causes epidemics of respiratory diseases in humans leading to thousands of death annually. One of its major virulence factors, the non-structural protein 1 (NS1), exhibits interferon-antagonistic properties. While epithelial cells of the respiratory tract are the primary targets of influenza virus, the virus-sensing mechanisms in these cells eventually leading to IFNbeta production are incompletely understood. Here we show that infection of epithelial cells with NS1-deficient influenza A virus upregulated expression of two molecules that have been previously implicated in sensing of RNA viruses, the retinoic acid-inducible gene I (RIG-I) and the melanoma differentiation-associated gene 5 (MDA5). Gene silencing and overexpression experiments demonstrated that RIG-I, its adapter interferon-beta promoter stimulator 1 (IPS-1) and interferon-regulated factor 3 (IRF3) were involved in influenza A virus-mediated production of the antiviral IFNbeta. In addition, we showed that the NS1 protein is capable to inhibit the RIG-I-induced signalling, a mechanism which corresponded to the observation that only NS1-deficient but not the wild-type virus induced high-level production of IFNbeta. In conclusion, we demonstrated a critical involvement of RIG-I, IPS-1 and IRF3 in influenza A virus infection of epithelial cells.