Change of oil-degrading activity in microorganisms stored under laboratory conditions

Change of the oil-degrading activity was studied in psychrophilic microbial strains Rhodococcus spp. DS-07, DS-21 and Pseudomonas spp. DS-09, DS-22 maintained on various media: rich and synthetic with a selective agent. After 2.5 years of storage on rich medium, the oil-degrading activity decreased...

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Bibliographic Details
Published inPrikladnaja biohimija i mikrobiologija Vol. 44; no. 3; p. 314
Main Authors Belousova, N I, Baryshnikova, L M, Shkidchenko, A N
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.2008
Subjects
Biodegradation, Environmental
Petroleum - metabolism
Plasmids - genetics
Plasmids - metabolism
Preservation, Biological - methods
Pseudomonas - genetics
Pseudomonas - growth & development
Rhodococcus - genetics
Rhodococcus - growth & development
Time Factors
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Summary:Change of the oil-degrading activity was studied in psychrophilic microbial strains Rhodococcus spp. DS-07, DS-21 and Pseudomonas spp. DS-09, DS-22 maintained on various media: rich and synthetic with a selective agent. After 2.5 years of storage on rich medium, the oil-degrading activity decreased by 50-60%, whereas this decrease was insignificant in the medium with oil. Passages to selective medium with oil after the storage partly restored the activity. It was found that storage of oil-degrading microorganisms caused loss of biodegradation plasmids. Their recovery and long-term preservation demand the presence of the selective agent in the medium.
ISSN:0555-1099